Homework 4 Us

  1. Which reading frame (5’3’) did you use to translate the sequence: 1, 2, or 3? _______ (2pt)
  • Where is the tag located: At the N-terminus or C-terminus of DHFR? ____________ (2pt)
  • Briefly describe how you found the tag location in (b.) above. (2pt)
  • What is the tag used: Myc, FLAG, GST, or His? _____________ (2pt)

What is the calculated molecular weight of this fusion protein? __________

Do you need help with your assignment?

An image as a link: W3Schools

  1. Which reading frame (5’3’) did you use to translate the sequence: 1, 2, or 3? _______ (2pt)
  • Where is the tag located: At the N-terminus or C-terminus of DHFR? ____________ (2pt)
  • Briefly describe how you found the tag location in (b.) above. (2pt)
  • What is the tag used: Myc, FLAG, GST, or His? _____________ (2pt)

What is the calculated molecular weight of this fusion protein? __________

Do you need help with your assignment?

An image as a link: W3Schools

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